The Food Microbiology Pathway: From Sample to Decision

Chromogenic Media: 

Highlight your food cultures in color!

Pathogens

Quality Indicators

Salmonella » Bacillus cereus »
Listeria » E.coli and Enterobacteriaceae »
Cronobacter sakazakii »  

 

Pathogens

Salmonella

ASAP™ Chromogenic Media

High quality media for Salmonella detection from food and environmental samples and for confirmation of VIDAS® UP Salmonella SPT.

AOAC-OMA approved for confirmation of VIDAS SPT positive assays

Labor time-saving method

  • Easy to differentiate with distinct pink-to-purple colonies for Salmonella on an opaque white agar
  • Reduces the need for subcultures

Reliable results

  • Results in 24 hours at 37˚C
  • High sensitivity and specificity
  • Inhibits interfering background flora in highly contaminated samples

 

Availability Packaging Reference
ASAP Media 20 Plates, 90mm AEB520090US

 

IBISA® Chromogenic Media

Highly selective media for motile and non-motile Salmonella spp. detection from food and environmental samples, and for confirmation of VIDAS® UP Salmonella SPT.

AOAC-OMA approved for confirmation of VIDAS SPT positive assays

  • IBISA principle is based on a mix of chromogenic substrates that produces a high level of differentiation between Salmonella and other Enterobacteriaceae.

Labor time-saving method

  • Easy to differentiate: green Salmonella colonies on a unique yellow agar
  • Background flora is inhibited and reduces the need for subcultures
  • Provides easy reading and reduces training needs

Reliable results

  • Results within 24 hours at 37˚C incubation

 

Availability Packaging Reference
IBISA Media 20 Plates, 90mm AEB520060
120 Plates, 90mm AEB520059

 

chromID® Salmonella Media

High quality chromogenic culture medium for selective isolation and differentiation of Salmonella spp. in food samples and for confirmation of VIDAS® Easy SLM.

AOAC-OMA approved for confirmation of VIDAS Easy SLM positive assays

Labor and time-saving method

  • Easy to differentiate with pink-to-mauve colonies for Salmonella on an opaque white agar
  • 3 chromogenic substrates optimize the selective isolation and differentiation of Salmonella
  • Reduces the need for subcultures

Reliable results

  • Results within 18 to 24 hours at 35-37˚C
  • Highly selective media ensures inhibition of contaminating food flora
  • High sensitivity minimizes false-negative results

 

Availability Packaging Reference
chromID Salmonella Media 20 Plates, 90mm 43621

 

Listeria

ALOA® Chromogenic Media

Selective media for the detection of Listeria monocytogenes and Listeria spp. in food and environmental samples and for confirmation for VIDAS® Listeria assays:

  • VIDAS UP Listeria (LPT)
  • VIDAS LMX
  • VIDAS LIS
  • VIDAS LM02

AOAC approved method for confirmation of VIDAS LPT, VIDAS LIS and VIDAS LMX-positive assays

Simple and reliable results

  • Rapid results in 24 hours at 37˚C
  • Easy-to-read: Listeria exhibits blue to blue-green colonies on an opaque background
    • No halo: Listeria spp.
    • With halo: L. monocytogenes

References

  • FDA BAM, Chapter 10
  • AOAC-RI and AOAC-OMA

 

Availability Packaging Reference
ALOA Medium 20 Plates, 90mm AEB520080
120 Plates, 90mm AEB520079
10 Plates, 140mm AEB120082
ALOA Agar Base - Dehydrated 500g AEB150072
ALOA Supplements 6 QSP 500ml AEB684420
ALOA Count Media Kit with Supplements QSP 5 x 200ml Bottles AEB620088

 

Cronobacter sakazakii

ESIA™ Chromogenic Media

Media for the detection of Cronobacter spp. (including Cronobacter sakazakii) in food samples such as milk powder, powdered infant formula and food prepared from milk powder.

ISO-compliant

  • ESIA is the ISO/TS 22964 standard required media for the detection of Cronobacter spp. in powdered milk and powdered infant formula.
  • Additional reagents required according to this ISO method are available: ESIA Agar, Buffered Peptone Water, mLST broth, TSA agar.

Easy-to-use

  • Blue-green colonies on a clear background
  • Convenient protocol "1bag/1plate": ESSB broth formulated to provide optimal growth of Cronobacter spp. while inhibiting interfering flora on ESIA agar
  • Results in 21 hours at 44˚C

 

Availability Packaging Reference
ESIA Media 20 Plates, 90mm AEB520010
ESIA Agar - Dehydrated 500g AEB150002
ESSB Broth - Cronobacter spp. 6 x 225 ml Bottles AEB611448

 

Quality Indicators 

Bacillus cereus

BACARA™ Chromogenic Media

New chromogenic culture medium for  detection and enumeration of Bacillus cereus

High Selectivity

  • No background flora growth
  • No cross reactions
  • Easy enumeration even with highly contaminated samples

High Specificity

  • Chromogenic medium utilizing enzymatic activity principle
  • Easy reading, even with difficult matrices
  • Possibility to enumerate Bacillus from the group cereus on acid or acidifying matrices

Easy Protocol (FDA BAM)

  • Spread up to 1ml on a 90 or 140mm plate, or pour-plate
  • Results within 24 hours

 

Availability Packaging Reference
BACARA Media 20 Plates, 90mm AEB520100
10 Plates, 140mm AEB120102
Kit, 5 Bottles (100ml + supplement) AEB620106

 

E.coli and Enterobacteriaceae

REBECCA™ Chromogenic Media

Media for the direct enumeration of E.coli and Enterobacteriaceae in food and water samples.

AFNOR validated method following ISO 16140 for both surface-innoculation and pour plate methods.

Flexible media (2 in one)
Two methods :

  • For enumeration of E.coli using REBECCA Base
  • For enumeration of E.coli and Enterobacteriaceae using REBECCA Base + EB Supplement

Easy-to-use

  • Results within 24 hours at 37˚C
  • Easy-to-read: blue colonies on clear agar for E.coli, pink-to-red colonies for other Enterobacteriaceae

 

Availability Packaging Reference
REBECCA Agar Base - Dehydrated 500g  AEB150022
REBECCA EB Media 20 Plates, 90mm                                        AEB520020
REBECCA Base 6 x 200ml Bottles     AEB620027
REBECCA EB Supplement 10 QSP 1.2L Bottle      AEB184135/10

 

Looking for more information on these chromogenic media? Contact us today.

ASAP Chromogenic Medium
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IBISA Chromogenic Medium
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chromID Salmonella Media
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ALOA is a trademark belonging to
Biolife Italiana S.r.l.

ESIA Chromogenic Media
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BACARA Chromogenic Media
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Reference: FDA BAM Chapter 14
"We concur with the AFNOR laboratories and suggest that Bacara replace MYP as a more rapid method to recover B. cereus from contaminated food products."

From Tallent et al.: Journal of AOAC International Vol. 95, No. 2, 2012

REBECCA Chromogenic Media
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media suite